The handling of Matrigel can
be tricky especially for first time users. You may find these tips and tricks
1.Thaw Matrigel at 4 °C
overnight (or at least 3 hours) before use. Matrigel
can be handled more easily when it is completely thawed.
2.Keep Matrigel on ice at all
times as Matrigel starts polymerizing quickly at room
temperature. Chilled pipette tips help reduce the coating of Matrigel on the surface of the tips and delay the
polymerization during pipetting.
3.Do not dilute Matrigel by
more than 50% (or less than 5 mg/ml) if it is to be used to form a 3D matrix in
the gel channel. Matrigel at a concentration of 10
mg/ml to 12 mg/ml can be used without dilution to form a 3D matrix in AIM
4.Fill Matrigel into the gel
channel as rapidly as possible to avoid the blocking of channel by polymerized
gel, especially if the Matrigel is used undiluted.
Do you have your own tips and tricks for handling Matrigel? Share them with the user community!